Natalia P. Malikova

Laboratory of Photobiology

Institute of Biophysics

Akademgorodok 660036 Krasnoyarsk Russia

Tel: +7 (391) 249-44-30

E-mail: npmal@yandex.ru

EDUCATIONAL BACKGROUND

• Ph.D. thesis The role of active site Trp residues in bioluminescent reaction of Ca2+-regulated photoprotein obelin

• 2004 Ph.D., Biophysics, Krasnoyarsk State University, Krasnoyarsk, Russia

• 1999 M.Sc., Biochemistry, Krasnoyarsk State University, Krasnoyarsk, Russia

PROFESSIONAL EXPERIENCE

• 2004-present Laboratory of Photobiology, Institute of Biophysics SB RAS, research scientist, junior research scientist (2003-2004)

INTERNATIONAL PROFESSIONAL TRAVEL

• 2007 (February-April) Collaborative research with the University of Wageningen, Wageningen, The Netherlands

INTERNATIONAL CONFERENCES/SEMINARS ATTENDED

• 2014 International Symposium on Bioluminescence and Chemiluminescence, Uppsala, Sweden

• 2014 1st AIST International Imaging Workshop, Tsukuba, Japan

• 2008 15th Int. Symposium on Bioluminescence and Chemiluminescence, Shanghai, China

• 2006 International Symposium “EU-Russia: Prospects for Cooperation in Biotechnology in the Seventh Framework Programme”, Saint Petersburg, Russia

• 2004 13th International Symposium on Bioluminescence and Chemiluminescence, Yokohama, Japan

• 2004 School-conference for young scientists, Vladivostok, Russia

PUBLICATIONS

PAPERS IN PEER REVIEWD JOURNALS

1. Markova S.V, Malikova N.P., Vysotski E.S., Frank L.A., Gitelson I.I. Bioluminescent monitoring enables observation of intracellular events in real time without cell and tissue destruction. Biophysics, 2017, 62, No. 3, 503-507.

2. Burakova L.P., Natashin P.V., Malikova N.P., Niu F., Pu M., Vysotski E.S., Liu Z.J. All Ca2+-binding loops of light-sensitive ctenophore photoprotein berovin bind magnesium ions: The spatial structure of Mg2+-loaded apo-berovin. J. Photochem. Photobiol. B. 2016, 154, 57-66.

3. Burakova L.P., Natashin P.V., Markova S.V., Eremeeva E.V., Malikova N.P., Cheng C., Liu Z.J., Vysotski E.S. Mitrocomin from the jellyfish Mitrocoma cellularia with deleted C-terminal tyrosine reveals a higher bioluminescence activity compared to wild type photoprotein. J. Photochem. Photobiol. B. 2016, 162, 286-297.

4. Malikova, N.P., Borgdorff, A.J., and Vysotski, E.S. Semisynthetic photoprotein reporters for tracking fast Ca2+ transients. Photochem. Photobiol. Sci., 2015, 2015, 14, 2213-2224.

5. Malikova, N.P., Burakova, L.P., Markova, S.V., Vysotski, E.S. Characterization of hydromedusan Ca(2+)-regulated photoproteins as a tool for measurement of Ca(2+)concentration. Anal. Bioanal. Chem., 2014, 406, 5715–5726.

6. Gealageas, R., Malikova, N.P., Picaud, S., Borgdorff, A.J., Burakova, L.P., Brulet, Ph., Vysotski, E.S., Dodd, R.H. Bioluminescent properties of obelin and aequorin with novel coelenterazine analogues. Anal. Bioanal. Chem., 2014, 406, 2695–2707.

7. Markova, S.V., Burakova, L.P., Golz, S., Malikova, N.P., Frank, L.A., Vysotski, E.S. The light-sensitive photoprotein berovin from the bioluminescent ctenophore Beroe abyssicola: a novel type of Ca(2+) -regulated photoprotein. FEBS J., 2012, 279, 856-870.

8. Malikova, N.P., Visser, N.V., van Hoek, A., Skakun, V.V., Vysotski, E.S., Lee, J., Visser, A.J. Green-fluorescent protein from the bioluminescent jellyfish Clytia gregaria is an obligate dimer and does not form a stable complex with the Ca2+-discharged photoprotein clytin. Biochemistry, 2011, 50, 4232-4241.

9. Stepanyuk, G.A., Unch, J., Malikova, N.P., Markova, S.V., Lee, J., Vysotski, E.S. Coelenterazine-v ligated to Ca2+-triggered coelenterazine-binding protein is a stable and efficient substrate of the red-shifted mutant of Renilla muelleri luciferase. Anal Bioanal Chem., 2010, 398, 1809-1817.

10. van Oort, B., Eremeeva, E.V., Koehorst, R.B., Laptenok, S.P., van Amerongen, H., van Berkel, W.J., Malikova, N.P., Markova, S.V., Vysotski, E.S., Visser, A.J., Lee, J. Picosecond fluorescence relaxation spectroscopy of the calcium-discharged photoproteins aequorin and obelin. Biochemistry, 2009, 48, 10486-10491.

11. Titushin, M.S., Markova, S.V., Frank, L.A., Malikova, N.P., Stepanyuk, G.A., Lee, J., Vysotski, E.S. Coelenterazine-binding protein of Renilla muelleri: cDNA cloning, overexpression, and characterization as a substrate of luciferase. Photochemical & photobiological sciences, 2008, 7, 189-196.

12. Frank, L.A., Borisova, V.V., Markova, S.V., Malikova, N.P., Stepanyuk, G.A., Vysotski, E.S. Violet and greenish photoprotein obelin mutants for reporter applications in dual-color assay. Analytical and bioanalytical chemistry, 2008, 391, 2891-2896.

13. Zhi-Jie Liu, G.A. Stepanyuk, E.S.Vysotski, J. Lee, S.V. Markova, N.P. Malikova, Bi-Cheng Wang. Crystal structure of obelin after Ca2+-triggered bioluminescence suggests neutral coelenteramide as the primary excited state // PNAS, 2006, V.103, pp.2570-2575

14. Malikova, N.P., Stepanyuk, G.A., Frank, L.A., Markova, S.V., Vysotski, E.S., Lee, J. Structural tuning of obelin bioluminescence by mutations of Trp92. FEBS Letters, 2003, 554, 184-188.

15. Vysotski, E.S., Liu, Zhi-Jie, Markova, S.V., Blinks, J.R., Deng, Lu , Frank, L.A., Herko, M., Malikova, N.P., Rose, J.P., Wang, Bi-Cheng, Lee, J. Violet bioluminescence and fast kinetics from W92F obelin: Structure-Based proposals for the bioluminescence triggering and the identification of the emitting species. Biochemistry, 2003, 42, 6013-6024.

16. Bondar, V.S., Purtov, K.V., Malikova, N.P., Frank, L.A., Illarionov B.A. Role of conservative residue Cys158 in the formation of an active photoprotein complex of obelin. Biochemistry, 2001, 66, 1014-1018.

17. Deng, L., Vysotski, E.S., Liu, Z.-J., Markova, S.V., Malikova, N.P., Lee, J., Rose, J., Wang, B.-C. Structural basis for the emission of violet bioluminescence from a W92F obelin mutant. FEBS Lett., 2001, 506, 281-285.

18. Bondar, V.S., Frank, L.A., Malikova, N.P., Inzhevatkin, E.V., Illarionova, V.A., Vysotski, E.S. Bioluminescent activity of the recombinant obelin after chemical modification of histidine and cysteine residues. In: Bioluminescence and Chemiluminescence: Perspectives for the 21st Century (Roda, A., Pazzagli, M., Kricka, L.J., and Stanley, P.E., Eds.), 1998, pp.400-403, John Wiley, NY.

PATENTS

• Маликова Н.П., Высоцкий Е.С., Маркова С.В., Степанюк Г.А. РФ № 2008145953/10 (060054) "Мутантный фотопротеин (варианты) для определения внутриклеточного кальция одновременно в разных органеллах клетки" от 27 октября 2010 г.

• Golz S, Vysotski E, Markova S, Malikova N, Frank L. «Isolated photoprotein mtclytin decay and the use thereof», WO/2008/107104, 12-SEP-2008