Laboratories

PHOTOBIOLOGY LABORATORY

Founded in 1961

Phone: +7 391 249 44 30

E-mail: eugene.vysotski@gmail.com

Head of the laboratory: Yevgeny Stepanovich Vysotsky, Ph.D.

Laboratory staff:

• Nadezhda S. Kudryasheva, D.Sc.

• Valentina A. Kratasyuk, D.Sc.

• Svetlana V. Markova, Ph.D.

• Valentin N. Petushkov, Ph.D.

• Lyudmila P. Burakova

• Elena V. Eremeeva, Ph.D.

• Elena N. Esimbekova, Ph.D.

• Natalia S. Rodionova, Ph.D.

• Marina D. Larionova

• Natalia P. Malikova, Ph.D.

• Pavel V. Natashin

• Elena V. Nemtseva, Ph.D.

• Viktoria I. Lonshakova-Mukina, Ph.D.

• Ekaterina S. Kovel

• Nadezhda I. Veryugina

• Olga V. Kolesnik

• Elizaveta M. Kolosova

• Igor A. Korotov

• Galina G. Eremich

• Maria O. Vyatkina

• Anastasia G. Seliverstova

List of major publications

1. Eremeeva E.V., Markova S.V., van Berkel W.J.H., Vysotski E.S. Role of key residues of obelin in coelenterazine binding and conversion into 2-hydroperoxy adduct // J. Photochem. Photobiol. B, 2013, 127:133-139.

2. Eremeeva EV, Markova SV, Frank LA, Visser AJ, van Berkel WJ, Vysotski ES. Bioluminescent and spectroscopic properties of His-Trp-Tyr triad mutants of obelin and aequorin // Photochem Photobiol Sci. 2013, 12(6):1016-24.

3. Eremeeva, E.V., Markova, S.V., Vysotski, E.S. Highly active BRET-reporter based on yellow mutant of Renilla muelleri luciferase // Dokl. Biochem. Biophys., 2013, 450:147-150.

4. Eremeeva, E.V., Natashin, P.V., Song, L., Zhou, Y.G., van Berkel, W.J.H., Liu, Z.J, Vysotski, E.S. Oxygen activation of apo-obelin-coelenterazine complex // Chembiochem., 2013, 14:739-745.

5. Esimbekova E., Kondik A., Kratasyuk V. Bioluminescent enzymatic rapid assay of water integral toxicity // Environmental Monitoring and Assessment, 2013. 185(7):5909-5916.

6. Krasitskaya V.V., Kudryavtsev A.N., Shimomura O., Frank L.A. Obelin mutants as reporters in bioluminescent dual-analyte binding assay // Anal. Methods, 2013, 5:636–640.

7. Roza R. Alieva, Nadezhda V. Belogurova, Alena S. Petrova, Nadezhda S. Kudryasheva. Fluorescence properties of Ca2+-independent discharged obelin and its application prospect // Anal.&Bioanal.Chem. 2013, 405(10):3351-3358.

8. Selivanova M.A., Mogilnaya O.A., Badun G.A., Vydryakova G.A., Kuznetsov A.M., Kudryasheva N.S. Effect of tritium on luminous marine bacteria and enzyme reactions // Journal of Environmental Radioactivity, 2013, 130:19-25.

9. Eremeeva, E.V., Vysotski, E.S., Westphal, A.H., van Mierlo, C.P.M., van Berkel, W.J.H. Ligand binding and conformational states of the photoprotein obelin // FEBS Lett., 2012, 586:4173–4179.

10. Kamnev A.A., Tugarova A.V., Selivanova M.A , Tarantilis P.A., Polissiou M.G., Kudryasheva N.S. Effects of americium-241 and humic substances on Photobacterium phosphoreum: bioluminescence and diffuse reflectance FTIR spectroscopic studies // Spectrochim. Acta A: Mol. Biomol. Spectrosc., 2012, 586(23):4173-4179.

11. Kudryavtsev A. N., Krasitskaya V.V., Petunin A.I., Burakov A.Y., Frank L.A. Simultaneous bioluminescent immunoassay of serum total and IgG-bound prolactins // Anal. Chem. 2012, 84:3119-3124.

12. Markova S.V., Burakova L.P., Golz S., Malikova N.P., Frank L.A., Vysotski E.S. The light-sensitive photoprotein berovin from the bioluminescent ctenophore Beroe abyssicola: a novel type of Ca2+-regulated photoprotein // FEBS J., 2012, 279(5):856-870.

13. Markova S.V., Burakova L.P., Vysotski E.S. High-active truncated luciferase of copepod Metridia longa // Biochem. Biophys. Res. Commun., 2012, 417(1):98-103.

14. Tarasova A.S., Kislan S.L., Fedorova E.S., Kuznetsov A.M., Mogilnaya O.A., Stom D.I., Kudryasheva N.S. Bioluminescence as a tool for studying detoxification processes in metal salt solutions involving humic substances // Journal of Photochemistry and Photobiology, 2012, 117(5):164–170.

15. Alexandrova M., Rozhko T., Vydryakova G., Kudryasheva N. Effect of americium-241 on luminous bacteria. Role of peroxides // Journal of Environmental Radioactivity, 2011, 102:407-411.

16. Kirillova Т.N., Gerasimova M.A., Nemtseva E.V., Kudryasheva N.S. Effect of halogenated fluorescent compounds on bioluminescent reaction // Anal.&Bioanal.Chem. 2011, 400(2):343-51.

17. Krasitskaya V. V., Korneeva S. I., Kudryavtsev A. N., Markova S. V., Stepanyuk G. A., Frank L. A. Ca2+-triggered coelenterazine-binding protein from Renilla as an enzyme-dependent label for binding assay // Anal. Bioanal. Chem., 2011, 401:2573–2579.

18. Kratasyuk V., Esimbekova E., Correll M., Bucklin R. Bioluminescent enzyme assay for the indication of plant stress in enclosed life support systems // Luminescence. 2011.

19. Malikova NP, Visser NV, van Hoek A, Skakun VV, Vysotski ES, Lee J, Visser AJ. Green-fluorescent protein from the bioluminescent jellyfish Clytia gregaria is an obligate dimer and does not form a stable complex with the Ca(2+)-discharged photoprotein clytin // Biochemistry, 2011, 50(20):4232-41.

20. Rozhko T.V., Bondareva L.G., Mogilnaya O.A., Vydryakova G.A., Bolsunovsky A.Ya., Stom D.I., Kudryasheva N.S. Detoxification of AM-241 Solutions by Humic Substances: Bioluminescent Monitoring // Anal.&Bioanal.Chem., 2011, 400(2):329–334.

21. Tarasova A.S. Stom D.I., Kydryasheva N.S. Bioluminescent toxicity monitoring of oxidizer solutions: effect of humic substances // Environmental Toxicology and Chemistry, 2011, 30(5):1013-7.

22. Belogurova N., Kudryasheva N. Discharged Photoprotein Obelin: Fluorescence Spectra Peculiarities // J. Photochem. Photobiol. B., 2010, 101:103-8.

23. Belogurova N., Kudryasheva N. Discharged Photoprotein Obelin: Fluorescence Peculiarities // J. Photochem. Photobiol. B., 2010, 101:103–108.

24. Eremeeva E. V., Frank L. A., Markova S. V., Vysotski E. Ca2+-regulated photoprotein obelin as N-terminal partner in the fusion proteins // Journal of SFU. Biology, 2010, 4:372-383.

25. Frank L. A., Ca2+-regulated photoproteins: effective immunoassay reporters // Sensors, 2010, 10:11287-11300.

26. Kratasyuk V., Esimbekova E., Correll M., Bucklin R. Bioluminescent enzyme assay for the indication of plant stress in enclosed life support systems // Luminescence, 2010.

27. Markova S. V., Burakova L. P., Frank L.A., Golz S., Korostileva K. A., Vysotski ES Green-fluorescent protein from the bioluminescent jellyfish Clytia gregaria cDNA cloning, expression, and characterization of novel recombinant protein // Photochem. Photobiol. Sci., 2010, 9:757–765.

28. Belogurova N.V., Kudryasheva N.S., Alieva R.R. Activity of upper electron-excited states in bioluminescence of coelenterates. // Journal of Molecular Structure, 2009, 924–926:148–152.

29. Vetrova Elena V., Kudryasheva Nadezhda S., Cheng Kwan H. Effect of quinone on the fluorescence decay dynamics of endogenous flavin bound to bacterial luciferase // Biophys. Chem, 2009, 141(1):59-65.